Multiplexed CRISPR/Cas9 mutagenesis of rice PSBS1 non-coding sequences for transgene-free overexpression

Former Ph.D. student Dhruv Patel-Tupper led this study published in Science Advances showing that unbiased promoter editing can be used to generate overexpression alleles in a crop plant. This is the first step towards non-transgenic VPZ crops with higher photosynthetic efficiency and yield!